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KMID : 1147820180070010020
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Journal of Naturopathy
2018 Volume.7 No. 1 p.20 ~ p.25
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Analysis of Thin Aggregative Fimbriae Genes csgA, csgB of S. typhimurium and S. enteritidis Strains
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Na Hun-Taek
Joung Maeng-Sig
Kim Hong-Seon
Kim Soo-Young
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Abstract
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The purpose of this study was investigated to observe the relatively low amino acid mutations in six different strains of ATCC or KCTC Salmonella strains (four species and two isolates). The mutations in the strains were to be detected and compared with the genes csgA and csgB corresponding to the Thin aggregative fimbriae. Amino acid mutations in the strains were observed from Ser20→ Gly(AGT→GGT) in the csgA gene, Asp25→Ala(GAT→GCT) and Lys66→Thr(AAA→ACA) in the csgB gene. Among the six strains, the two most common amino acid variations were observed in S. typhimurium - TH strains. On the other hand, no mutation of nucleotide sequence was observed in the strains of S. typhimurium ATCC 13311 and S. typhimurium KCTC 1925. In conclusion the genes csgA and csgB in the strains may be useful for the evaluation and detection of amino acid mutation.
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KEYWORD
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Mutation, S. typhimurium, S. enteritidis, gene csgA, gene csgB
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